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Rapid identification and expression of human TCRs in retrogenic mice()

Single-cell paired TCR identification is a powerful tool, but has been limited in its previous incompatibility with further functional analysis. The current protocol describes a method to clone and functionally evaluate in vivo TCRs derived from single antigen-responsive human T cells and monoclonal...

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Bibliographische Detailangaben
Veröffentlicht in:J Immunol Methods
Hauptverfasser: Sprouse, Maran L., Blahnik, Gabriele, Lee, Thomas, Tully, Natalie, Banerjee, Pinaki, James, Eddie A., Redondo, Maria J., Bettini, Matthew L., Bettini, Maria
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5439214/
https://ncbi.nlm.nih.gov/pubmed/27589924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2016.08.010
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