Llwytho...
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
Although, several methods for sequencing of paired T cell receptor (TCR) alpha and beta chains from single T cells have been developed, none so far have been conducive to downstream in vivo functional analysis of TCR heterodimers. We have developed an improved protocol based on a two-step multiplex-...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | J Vis Exp |
|---|---|
| Prif Awduron: | , , , , , , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
MyJove Corporation
2017
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5752194/ https://ncbi.nlm.nih.gov/pubmed/28930975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55379 |
| Tagiau: |
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