Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Although, several methods for sequencing of paired T cell receptor (TCR) alpha and beta chains from single T cells have been developed, none so far have been conducive to downstream in vivo functional analysis of TCR heterodimers. We have developed an improved protocol based on a two-step multiplex-...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:J Vis Exp
Egile Nagusiak: Sprouse, Maran L., Blahnik, Gabriele, Lee, Thomas, Tully, Natalie, Benarjee, Pinaki, James, Eddie A., Redondo, Maria J., Bettini, Matthew L., Bettini, Maria
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: MyJove Corporation 2017
Gaiak:
Immunology
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752194/
https://ncbi.nlm.nih.gov/pubmed/28930975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55379
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