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Systematic mapping of functional enhancer-promoter connections with CRISPR interference

Gene expression in mammals is regulated by noncoding elements that can impact physiology and disease, yet the functions and target genes of most noncoding elements remain unknown. We present a high-throughput approach that uses CRISPR interference (CRISPRi) to discover regulatory elements and identi...

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Publicat a:Science
Autors principals: Fulco, Charles P., Munschauer, Mathias, Anyoha, Rockwell, Munson, Glen, Grossman, Sharon R., Perez, Elizabeth M., Kane, Michael, Cleary, Brian, Lander, Eric S., Engreitz, Jesse M.
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5438575/
https://ncbi.nlm.nih.gov/pubmed/27708057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.aag2445
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