Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

BACKGROUND: Plasmodium gametocytes are sexual stages transmitted to female Anopheles mosquitoes. While Plasmodium parasites can be differentiated microscopically on Giemsa-stained blood smears, molecular methods are increasingly used because of their increased sensitivity. Molecular detection of gam...

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Detalhes bibliográficos
Publicado no:Malar J
Main Authors: Hanron, Amelia E., Billman, Zachary P., Seilie, Annette M., Olsen, Tayla M., Fishbaugher, Matthew, Chang, Ming, Rueckle, Thomas, Andenmatten, Nicole, Greenhouse, Bryan, Arinaitwe, Emmanuel, Rek, John, Das, Smita, Domingo, Gonzalo J., Shipman, Kelly, Kappe, Stefan H., Kublin, James G., Murphy, Sean C.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5438523/
https://ncbi.nlm.nih.gov/pubmed/28526032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12936-017-1863-3
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