Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

BACKGROUND: Plasmodium gametocytes are sexual stages transmitted to female Anopheles mosquitoes. While Plasmodium parasites can be differentiated microscopically on Giemsa-stained blood smears, molecular methods are increasingly used because of their increased sensitivity. Molecular detection of gam...

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Udgivet i:Malar J
Main Authors: Hanron, Amelia E., Billman, Zachary P., Seilie, Annette M., Olsen, Tayla M., Fishbaugher, Matthew, Chang, Ming, Rueckle, Thomas, Andenmatten, Nicole, Greenhouse, Bryan, Arinaitwe, Emmanuel, Rek, John, Das, Smita, Domingo, Gonzalo J., Shipman, Kelly, Kappe, Stefan H., Kublin, James G., Murphy, Sean C.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2017
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5438523/
https://ncbi.nlm.nih.gov/pubmed/28526032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12936-017-1863-3
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