Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

BACKGROUND: Plasmodium gametocytes are sexual stages transmitted to female Anopheles mosquitoes. While Plasmodium parasites can be differentiated microscopically on Giemsa-stained blood smears, molecular methods are increasingly used because of their increased sensitivity. Molecular detection of gam...

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Dettagli Bibliografici
Pubblicato in:Malar J
Autori principali: Hanron, Amelia E., Billman, Zachary P., Seilie, Annette M., Olsen, Tayla M., Fishbaugher, Matthew, Chang, Ming, Rueckle, Thomas, Andenmatten, Nicole, Greenhouse, Bryan, Arinaitwe, Emmanuel, Rek, John, Das, Smita, Domingo, Gonzalo J., Shipman, Kelly, Kappe, Stefan H., Kublin, James G., Murphy, Sean C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2017
Soggetti:
Research
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5438523/
https://ncbi.nlm.nih.gov/pubmed/28526032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12936-017-1863-3
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