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The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro

As Cpf1 cleaves double-stranded DNA in a staggered way, it can be used in DNA assembly. However, the Cpf1 cleavage was found to be inaccurate, which may cause errors in DNA assembly. Here, the Cpf1 cleavage sites were precisely characterized, where the cleavage site on the target strand was around t...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Lei, Chao, Li, Shi-Yuan, Liu, Jia-Kun, Zheng, Xuan, Zhao, Guo-Ping, Wang, Jin
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5436000/
https://ncbi.nlm.nih.gov/pubmed/28115632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx018
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