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CRISPR/Cpf1-mediated DNA-free plant genome editing

Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe the delivery of...

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Detalles Bibliográficos
Publicado en:Nat Commun
Main Authors: Kim, Hyeran, Kim, Sang-Tae, Ryu, Jahee, Kang, Beum-Chang, Kim, Jin-Soo, Kim, Sang-Gyu
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5316869/
https://ncbi.nlm.nih.gov/pubmed/28205546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms14406
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