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A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein Engineering
Directed evolution is a powerful tool for optimizing enzymes, and mutagenesis methods that improve enzyme library quality can significantly expedite the evolution process. Here, we report a simple method for targeted combinatorial codon mutagenesis (CCM). To demonstrate the utility of this method fo...
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| Vydáno v: | ACS Synth Biol |
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| Hlavní autoři: | , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2017
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5435451/ https://ncbi.nlm.nih.gov/pubmed/28033708 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.6b00297 |
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