Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

Chemical modification of the gRNA and donor DNA has great potential for improving the gene editing efficiency of Cas9 and Cpf1, but has not been investigated extensively. In this report, we demonstrate that the gRNAs of Cas9 and Cpf1, and donor DNA can be chemically modified at their terminal positi...

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Bibliografiska uppgifter
I publikationen:eLife
Huvudupphovsmän: Lee, Kunwoo, Mackley, Vanessa A, Rao, Anirudh, Chong, Anthony T, Dewitt, Mark A, Corn, Jacob E, Murthy, Niren
Materialtyp: Artigo
Språk:Inglês
Publicerad: eLife Sciences Publications, Ltd 2017
Ämnen:
Genes and Chromosomes
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5413346/
https://ncbi.nlm.nih.gov/pubmed/28462777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.25312
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