A Combination of CRISPR/Cas9 and Standardized RNAi as a Versatile Platform for the Characterization of Gene Function

Traditional loss-of-function studies in Drosophila suffer from a number of shortcomings, including off-target effects in the case of RNA interference (RNAi) or the stochastic nature of mosaic clonal analysis. Here, we describe minimal in vivo GFP interference (miGFPi) as a versatile strategy to char...

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Vydáno v:G3 (Bethesda)
Hlavní autoři: Wissel, Sebastian, Kieser, Anja, Yasugi, Tetsuo, Duchek, Peter, Roitinger, Elisabeth, Gokcezade, Joseph, Steinmann, Victoria, Gaul, Ulrike, Mechtler, Karl, Förstemann, Klaus, Knoblich, Jürgen A., Neumüller, Ralph A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Genetics Society of America 2016
Témata:
Investigations
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4978900/
https://ncbi.nlm.nih.gov/pubmed/27280787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.116.028571
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