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Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila

The CRISPR-associated RNA-guided nuclease Cas9 has emerged as a powerful tool for genome engineering in a variety of organisms. To achieve efficient gene targeting rates in Drosophila, current approaches require either injection of in vitro transcribed RNAs or injection into transgenic Cas9-expressi...

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Detalhes bibliográficos
Publicado no:G3 (Bethesda)
Main Authors: Gokcezade, Joseph, Sienski, Grzegorz, Duchek, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4232553/
https://ncbi.nlm.nih.gov/pubmed/25236734
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.114.014126
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