Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells

Loss-of-function studies in human pluripotent stem cells (hPSCs) require efficient methodologies for lesion of genes of interest. Here, we introduce a donor-free paired gRNA-guided CRISPR/Cas9 knockout strategy (paired-KO) for efficient and rapid gene ablation in hPSCs. Through paired-KO, we succeed...

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Detalhes bibliográficos
Publicado no:Stem Cell Reports
Main Authors: Liu, Zhongliang, Hui, Yi, Shi, Lei, Chen, Zhenyu, Xu, Xiangjie, Chi, Liankai, Fan, Beibei, Fang, Yujiang, Liu, Yang, Ma, Lin, Wang, Yiran, Xiao, Lei, Zhang, Quanbin, Jin, Guohua, Liu, Ling, Zhang, Xiaoqing
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5032288/
https://ncbi.nlm.nih.gov/pubmed/27594587
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2016.07.021
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