Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

In Parkinson's Disease (PD) there is widespread neuronal loss throughout the brain with pronounced degeneration of dopaminergic neurons in the SNc, leading to bradykinesia, rigidity, and tremor. The identification of living dopaminergic neurons in primary Ventral Mesencephalic (VM) cultures usi...

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Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Henley, Beverley M., Cohen, Bruce N., Kim, Charlene H., Gold, Heather D., Srinivasan, Rahul, McKinney, Sheri, Deshpande, Purnima, Lester, Henry A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2017
Konular:
Neuroscience
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5409190/
https://ncbi.nlm.nih.gov/pubmed/28287593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54981
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