Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

In Parkinson's Disease (PD) there is widespread neuronal loss throughout the brain with pronounced degeneration of dopaminergic neurons in the SNc, leading to bradykinesia, rigidity, and tremor. The identification of living dopaminergic neurons in primary Ventral Mesencephalic (VM) cultures usi...

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Vydáno v:J Vis Exp
Hlavní autoři: Henley, Beverley M., Cohen, Bruce N., Kim, Charlene H., Gold, Heather D., Srinivasan, Rahul, McKinney, Sheri, Deshpande, Purnima, Lester, Henry A.
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2017
Témata:
Neuroscience
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5409190/
https://ncbi.nlm.nih.gov/pubmed/28287593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54981
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