Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

In Parkinson's Disease (PD) there is widespread neuronal loss throughout the brain with pronounced degeneration of dopaminergic neurons in the SNc, leading to bradykinesia, rigidity, and tremor. The identification of living dopaminergic neurons in primary Ventral Mesencephalic (VM) cultures usi...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Henley, Beverley M., Cohen, Bruce N., Kim, Charlene H., Gold, Heather D., Srinivasan, Rahul, McKinney, Sheri, Deshpande, Purnima, Lester, Henry A.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Neuroscience
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5409190/
https://ncbi.nlm.nih.gov/pubmed/28287593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54981
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