Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

In Parkinson's Disease (PD) there is widespread neuronal loss throughout the brain with pronounced degeneration of dopaminergic neurons in the SNc, leading to bradykinesia, rigidity, and tremor. The identification of living dopaminergic neurons in primary Ventral Mesencephalic (VM) cultures usi...

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書目詳細資料
發表在:J Vis Exp
Main Authors: Henley, Beverley M., Cohen, Bruce N., Kim, Charlene H., Gold, Heather D., Srinivasan, Rahul, McKinney, Sheri, Deshpande, Purnima, Lester, Henry A.
格式: Artigo
語言:Inglês
出版: MyJove Corporation 2017
主題:
Neuroscience
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5409190/
https://ncbi.nlm.nih.gov/pubmed/28287593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54981
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