Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Induced Pluripotent Stem Cells (iPSCs) hold great promise for disease modeling and regenerative therapies. We previously reported the use of Episomal Vectors (EV) to generate integration-free iPSCs from peripheral blood mononuclear cells (PB MNCs). The episomal vectors used are DNA plasmids incorpor...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Wen, Wei, Zhang, Jian-Ping, Chen, Wanqiu, Arakaki, Cameron, Li, Xiaolan, Baylink, David, Botimer, Gary D., Xu, Jing, Yuan, Weiping, Cheng, Tao, Zhang, Xiao-Bing
Formato: Artigo
Idioma:Inglês
Publicado: MyJove Corporation 2017
Assuntos:
Developmental Biology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408725/
https://ncbi.nlm.nih.gov/pubmed/28117800
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55091
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