Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Induced Pluripotent Stem Cells (iPSCs) hold great promise for disease modeling and regenerative therapies. We previously reported the use of Episomal Vectors (EV) to generate integration-free iPSCs from peripheral blood mononuclear cells (PB MNCs). The episomal vectors used are DNA plasmids incorpor...

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Enregistré dans:
Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Wen, Wei, Zhang, Jian-Ping, Chen, Wanqiu, Arakaki, Cameron, Li, Xiaolan, Baylink, David, Botimer, Gary D., Xu, Jing, Yuan, Weiping, Cheng, Tao, Zhang, Xiao-Bing
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2017
Sujets:
Developmental Biology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408725/
https://ncbi.nlm.nih.gov/pubmed/28117800
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55091
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