Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Induced Pluripotent Stem Cells (iPSCs) hold great promise for disease modeling and regenerative therapies. We previously reported the use of Episomal Vectors (EV) to generate integration-free iPSCs from peripheral blood mononuclear cells (PB MNCs). The episomal vectors used are DNA plasmids incorpor...

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Dades bibliogràfiques
Publicat a:J Vis Exp
Autors principals: Wen, Wei, Zhang, Jian-Ping, Chen, Wanqiu, Arakaki, Cameron, Li, Xiaolan, Baylink, David, Botimer, Gary D., Xu, Jing, Yuan, Weiping, Cheng, Tao, Zhang, Xiao-Bing
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2017
Matèries:
Developmental Biology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408725/
https://ncbi.nlm.nih.gov/pubmed/28117800
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55091
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