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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Characterization of molecular interactions in terms of basic binding parameters such as binding affinity, stoichiometry, and thermodynamics is an essential step in basic and applied science. MicroScale Thermophoresis (MST) is a sensitive biophysical method to obtain this important information. Relyi...

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Publicado en:J Vis Exp
Autores principales: Entzian, Clemens, Schubert, Thomas
Formato: Artigo
Lenguaje:Inglês
Publicado: MyJove Corporation 2017
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408713/
https://ncbi.nlm.nih.gov/pubmed/28117825
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55070
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