Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis

MicroScale Thermophoresis (MST) is a frequently used method for the quantitative characterization of intermolecular interactions with several advantages over other technologies. One of these is its capability to determine equilibrium constants in solution including complex biological matrices such a...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Bartoschik, Tanja, Galinec, Stefanie, Kleusch, Christian, Walkiewicz, Katarzyna, Breitsprecher, Dennis, Weigert, Sebastian, Muller, Yves A., You, Changjiang, Piehler, Jacob, Vercruysse, Thomas, Daelemans, Dirk, Tschammer, Nuska
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5862892/
https://ncbi.nlm.nih.gov/pubmed/29563556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-23154-3
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