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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Characterization of molecular interactions in terms of basic binding parameters such as binding affinity, stoichiometry, and thermodynamics is an essential step in basic and applied science. MicroScale Thermophoresis (MST) is a sensitive biophysical method to obtain this important information. Relyi...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5408713/ https://ncbi.nlm.nih.gov/pubmed/28117825 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55070 |
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