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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Characterization of molecular interactions in terms of basic binding parameters such as binding affinity, stoichiometry, and thermodynamics is an essential step in basic and applied science. MicroScale Thermophoresis (MST) is a sensitive biophysical method to obtain this important information. Relyi...

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Enregistré dans:
Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Entzian, Clemens, Schubert, Thomas
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408713/
https://ncbi.nlm.nih.gov/pubmed/28117825
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55070
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