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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Characterization of molecular interactions in terms of basic binding parameters such as binding affinity, stoichiometry, and thermodynamics is an essential step in basic and applied science. MicroScale Thermophoresis (MST) is a sensitive biophysical method to obtain this important information. Relyi...
Enregistré dans:
| Publié dans: | J Vis Exp |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MyJove Corporation
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5408713/ https://ncbi.nlm.nih.gov/pubmed/28117825 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55070 |
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