Development of a high-titer retrovirus producer cell line capable of gene transfer into rhesus monkey hematopoietic stem cells.

Retroviral-mediated gene transfer into primitive hematopoietic cells has been difficult to achieve in large-animal models. We have developed an amphotropic producer clone that generates greater than 10(10) recombinant retroviral particles (colony-forming units) per ml of culture medium. Autologous r...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Bodine, D M, McDonagh, K T, Brandt, S J, Ney, P A, Agricola, B, Byrne, E, Nienhuis, A W
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53978/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2339117/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.10.3738
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