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An enhanced recombinant amino‐terminal acetylation system and novel in vivo high‐throughput screen for molecules affecting α‐synuclein oligomerisation

Amino‐terminal acetylation is a ubiquitous protein modification affecting the majority of eukaryote proteins to regulate stability and function. We describe an optimised recombinant expression system for rapid production of amino terminal‐acetylated proteins within bacteria. We go on to describe the...

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Detalhes bibliográficos
Publicado no:FEBS Lett
Main Authors: Eastwood, Tara A., Baker, Karen, Brooker, Holly R., Frank, Stefanie, Mulvihill, Daniel P.
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5396276/
https://ncbi.nlm.nih.gov/pubmed/28214355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1873-3468.12597
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