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An enhanced recombinant amino‐terminal acetylation system and novel in vivo high‐throughput screen for molecules affecting α‐synuclein oligomerisation

Amino‐terminal acetylation is a ubiquitous protein modification affecting the majority of eukaryote proteins to regulate stability and function. We describe an optimised recombinant expression system for rapid production of amino terminal‐acetylated proteins within bacteria. We go on to describe the...

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Publicado en:FEBS Lett
Autores principales: Eastwood, Tara A., Baker, Karen, Brooker, Holly R., Frank, Stefanie, Mulvihill, Daniel P.
Formato: Artigo
Lenguaje:Inglês
Publicado: John Wiley and Sons Inc. 2017
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5396276/
https://ncbi.nlm.nih.gov/pubmed/28214355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1873-3468.12597
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