Mechanism of transcription initiation and promoter escape by E. coli RNA polymerase

To investigate roles of the discriminator and open complex (OC) lifetime in transcription initiation by Escherichia coli RNA polymerase (RNAP; α(2)ββ’ωσ(70)), we compare productive and abortive initiation rates, short RNA distributions, and OC lifetime for the λP(R) and T7A1 promoters and variants w...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Henderson, Kate L., Felth, Lindsey C., Molzahn, Cristen M., Shkel, Irina, Wang, Si, Chhabra, Munish, Ruff, Emily F., Bieter, Lauren, Kraft, Joseph E., Record, M. Thomas
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2017
Matèries:
PNAS Plus
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5393250/
https://ncbi.nlm.nih.gov/pubmed/28348246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1618675114
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