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Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering
The CRISPR/Cas system, in which the Cas9 endonuclease and a guide RNA complementary to the target are sufficient for RNA-guided cleavage of the target DNA, is a powerful new approach recently developed for targeted gene disruption in various animal models. However, there is little verification of mi...
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| Udgivet i: | Sci Rep |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Nature Publishing Group
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5380110/ https://ncbi.nlm.nih.gov/pubmed/24675426 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04513 |
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