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Efficient generation of conditional knockout mice via sequential introduction of lox sites
Conditional knockout using Cre/lox is essential for functional analysis of genes. CRISPR/Cas in combination with two sets of guide RNAs and a single-stranded oligonucleotide enables simultaneous insertion of two lox sequences. However, this method induces double-strand breaks at two sites on the sam...
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| Publicado no: | Sci Rep |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5554182/ https://ncbi.nlm.nih.gov/pubmed/28801621 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-08496-8 |
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