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Rational mutagenesis by engineering disulphide bonds improves Kluyveromyces lactis beta-galactosidase for high-temperature industrial applications

Kluyveromyces lactis β-galactosidase (Kl-β-Gal) is one of the most important enzymes in the dairy industry. The poor stability of this enzyme limits its use in the synthesis of galactooligosaccharides (GOS) and other applications requiring high operational temperature. To obtain thermoresistant vari...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Rico-Díaz, Agustín, Álvarez-Cao, María-Efigenia, Escuder-Rodríguez, Juan-José, González-Siso, María-Isabel, Cerdán, M. Esperanza, Becerra, Manuel
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5374532/
https://ncbi.nlm.nih.gov/pubmed/28361909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep45535
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