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Rational mutagenesis by engineering disulphide bonds improves Kluyveromyces lactis beta-galactosidase for high-temperature industrial applications

Kluyveromyces lactis β-galactosidase (Kl-β-Gal) is one of the most important enzymes in the dairy industry. The poor stability of this enzyme limits its use in the synthesis of galactooligosaccharides (GOS) and other applications requiring high operational temperature. To obtain thermoresistant vari...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Rico-Díaz, Agustín, Álvarez-Cao, María-Efigenia, Escuder-Rodríguez, Juan-José, González-Siso, María-Isabel, Cerdán, M. Esperanza, Becerra, Manuel
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5374532/
https://ncbi.nlm.nih.gov/pubmed/28361909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep45535
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