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Genomic subtraction for cloning DNA corresponding to deletion mutations.

We have developed a technique, called genomic subtraction, for isolating the DNA that is absent in deletion mutants. The method removes from wild-type DNA the sequences that are present in both the wild-type and the deletion mutant genomes. The DNA that corresponds to the deleted region remains. Enr...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Straus, D, Ausubel, F M
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1990
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Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53589/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2408039/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.5.1889
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