Genomic subtraction for cloning DNA corresponding to deletion mutations.
We have developed a technique, called genomic subtraction, for isolating the DNA that is absent in deletion mutants. The method removes from wild-type DNA the sequences that are present in both the wild-type and the deletion mutant genomes. The DNA that corresponds to the deleted region remains. Enr...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1990
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53589/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2408039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.5.1889 |
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