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Genomic subtraction for cloning DNA corresponding to deletion mutations.

We have developed a technique, called genomic subtraction, for isolating the DNA that is absent in deletion mutants. The method removes from wild-type DNA the sequences that are present in both the wild-type and the deletion mutant genomes. The DNA that corresponds to the deleted region remains. Enr...

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Hlavní autoři: Straus, D, Ausubel, F M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC53589/
https://ncbi.nlm.nih.gov/pubmed/2408039
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