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Genomic subtraction for cloning DNA corresponding to deletion mutations.

We have developed a technique, called genomic subtraction, for isolating the DNA that is absent in deletion mutants. The method removes from wild-type DNA the sequences that are present in both the wild-type and the deletion mutant genomes. The DNA that corresponds to the deleted region remains. Enr...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Principais autores: Straus, D, Ausubel, F M
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1990
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53589/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2408039/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.5.1889
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