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Rapid analysis of CpG methylation patterns using RNase T1 cleavage and MALDI-TOF

Here, we introduce a method for the fast and accurate analysis of DNA methylation based on bisulfite-treated DNA. The target region is PCR amplified using a T7 RNA polymerase promoter-tagged primer. A subsequent in vitro transcription leads to a transcript which contains guanosine residues only at s...

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Bibliografski detalji
Izdano u:Nucleic Acids Res
Glavni autori: Schatz, Philipp, Dietrich, Dimo, Schuster, Matthias
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2004
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC535694/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15576674/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh165
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