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Rapid analysis of CpG methylation patterns using RNase T1 cleavage and MALDI-TOF
Here, we introduce a method for the fast and accurate analysis of DNA methylation based on bisulfite-treated DNA. The target region is PCR amplified using a T7 RNA polymerase promoter-tagged primer. A subsequent in vitro transcription leads to a transcript which contains guanosine residues only at s...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC535694/ https://ncbi.nlm.nih.gov/pubmed/15576674 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh165 |
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