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Rapid analysis of CpG methylation patterns using RNase T1 cleavage and MALDI-TOF
Here, we introduce a method for the fast and accurate analysis of DNA methylation based on bisulfite-treated DNA. The target region is PCR amplified using a T7 RNA polymerase promoter-tagged primer. A subsequent in vitro transcription leads to a transcript which contains guanosine residues only at s...
Gorde:
| Argitaratua izan da: | Nucleic Acids Res |
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| Egile Nagusiak: | , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Oxford University Press
2004
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC535694/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15576674/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh165 |
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