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Rapid analysis of CpG methylation patterns using RNase T1 cleavage and MALDI-TOF

Here, we introduce a method for the fast and accurate analysis of DNA methylation based on bisulfite-treated DNA. The target region is PCR amplified using a T7 RNA polymerase promoter-tagged primer. A subsequent in vitro transcription leads to a transcript which contains guanosine residues only at s...

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Detalhes bibliográficos
Main Authors: Schatz, Philipp, Dietrich, Dimo, Schuster, Matthias
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC535694/
https://ncbi.nlm.nih.gov/pubmed/15576674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh165
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