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Reevaluation and Reduction of a PCR Bias Caused by Reannealing of Templates
We reevaluated the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies and showed that the template reannealing at the annealing step would not cause the bias; however, the preferential homoduplex formation during temperature decrease from denaturation to annealing st...
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| Autors principals: | , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC535213/ https://ncbi.nlm.nih.gov/pubmed/15574958 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.12.7545-7549.2004 |
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