Bias in Template-to-Product Ratios in Multitemplate PCR

Bias introduced by the simultaneous amplification of specific genes from complex mixtures of templates remains poorly understood. To explore potential causes and the extent of bias in PCR amplification of 16S ribosomal DNAs (rDNAs), genomic DNAs of two closely and one distantly related bacterial spe...

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Bibliographic Details
Main Authors: Polz, Martin F., Cavanaugh, Colleen M.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1998
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC106531/
https://ncbi.nlm.nih.gov/pubmed/9758791
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