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Bias in Template-to-Product Ratios in Multitemplate PCR

Bias introduced by the simultaneous amplification of specific genes from complex mixtures of templates remains poorly understood. To explore potential causes and the extent of bias in PCR amplification of 16S ribosomal DNAs (rDNAs), genomic DNAs of two closely and one distantly related bacterial spe...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: Polz, Martin F., Cavanaugh, Colleen M.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1998
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106531/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9758791/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.10.3724-3730.1998
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