Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids

We had been unsuccessful to amplify desired nucleotide sequences from various environmental DNA samples by using the inverse polymerase chain reaction (IPCR) technique, most probably because the copy numbers of target DNA sequences had been quite low. To enrich the target DNA sequences prior to IPCR...

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Detalhes bibliográficos
Main Authors: Yamada, Kazutaka, Terahara, Takeshi, Kurata, Shinya, Yokomaku, Toyokazu, Tsuneda, Satoshi, Harayama, Shigeaki
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Publishing Ltd 2008
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2327201/
https://ncbi.nlm.nih.gov/pubmed/18093161
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1462-2920.2007.01518.x
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