Reevaluation and Reduction of a PCR Bias Caused by Reannealing of Templates

We reevaluated the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies and showed that the template reannealing at the annealing step would not cause the bias; however, the preferential homoduplex formation during temperature decrease from denaturation to annealing st...

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Hlavní autoři: Kurata, Shinya, Kanagawa, Takahiro, Magariyama, Yukio, Takatsu, Kyoko, Yamada, Kazutaka, Yokomaku, Toyokazu, Kamagata, Yoichi
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2004
Témata:
Methods
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC535213/
https://ncbi.nlm.nih.gov/pubmed/15574958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.12.7545-7549.2004
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