Broad-Host-Range Plasmid pJB658 Can Be Used for Industrial-Level Production of a Secreted Host-Toxic Single-Chain Antibody Fragment in Escherichia coli

In industrial scale recombinant protein production it is often of interest to be able to translocate the product to reduce downstream costs, and heterologous proteins may require the oxidative environment outside of the cytoplasm for correct folding. High-level expression combined with translocation...

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Udgivet i:Appl Environ Microbiol
Main Authors: Sletta, H., Nedal, A., Aune, T. E. V., Hellebust, H., Hakvåg, S., Aune, R., Ellingsen, T. E., Valla, S., Brautaset, T.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2004
Fag:
Physiology and Biotechnology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC535149/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15574897/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.12.7033-7039.2004
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