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Broad-Host-Range Plasmid pJB658 Can Be Used for Industrial-Level Production of a Secreted Host-Toxic Single-Chain Antibody Fragment in Escherichia coli

In industrial scale recombinant protein production it is often of interest to be able to translocate the product to reduce downstream costs, and heterologous proteins may require the oxidative environment outside of the cytoplasm for correct folding. High-level expression combined with translocation...

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Autors principals: Sletta, H., Nedal, A., Aune, T. E. V., Hellebust, H., Hakvåg, S., Aune, R., Ellingsen, T. E., Valla, S., Brautaset, T.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC535149/
https://ncbi.nlm.nih.gov/pubmed/15574897
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.12.7033-7039.2004
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