Broad-Host-Range Plasmid pJB658 Can Be Used for Industrial-Level Production of a Secreted Host-Toxic Single-Chain Antibody Fragment in Escherichia coli

In industrial scale recombinant protein production it is often of interest to be able to translocate the product to reduce downstream costs, and heterologous proteins may require the oxidative environment outside of the cytoplasm for correct folding. High-level expression combined with translocation...

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Detalhes bibliográficos
Main Authors: Sletta, H., Nedal, A., Aune, T. E. V., Hellebust, H., Hakvåg, S., Aune, R., Ellingsen, T. E., Valla, S., Brautaset, T.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Physiology and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC535149/
https://ncbi.nlm.nih.gov/pubmed/15574897
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.12.7033-7039.2004
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