The Presence of N-Terminal Secretion Signal Sequences Leads to Strong Stimulation of the Total Expression Levels of Three Tested Medically Important Proteins during High-Cell-Density Cultivations of Escherichia coli

Genetic optimizations to achieve high-level production of three different proteins of medical importance for humans, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon alpha 2b (IFN-α2b), and single-chain antibody variable fragment (scFv-phOx), were investigated during high-cell-d...

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Main Authors: Sletta, H., Tøndervik, A., Hakvåg, S., Aune, T. E. Vee, Nedal, A., Aune, R., Evensen, G., Valla, S., Ellingsen, T. E., Brautaset, T.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Physiology and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1800768/
https://ncbi.nlm.nih.gov/pubmed/17142370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01804-06
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