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Allelic Dropout During Polymerase Chain Reaction due to G-Quadruplex Structures and DNA Methylation Is Widespread at Imprinted Human Loci

Loss of one allele during polymerase chain reaction (PCR) amplification of DNA, known as allelic dropout, can be caused by a variety of mechanisms. Allelic dropout during PCR may have profound implications for molecular diagnostic and research procedures that depend on PCR and assume biallelic ampli...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
הוצא לאור ב:G3 (Bethesda)
Main Authors: Stevens, Aaron J., Taylor, Millie G., Pearce, Frederick Grant, Kennedy, Martin A.
פורמט: Artigo
שפה:Inglês
יצא לאור: Genetics Society of America 2017
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC5345703/
https://ncbi.nlm.nih.gov/pubmed/28143949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.116.038687
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