Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells

Wnt signalling proteins are essential for culture of human organ stem cells in organoids, but most Wnt protein formulations are poorly active in serum-free media. Here we show that purified Wnt3a protein is ineffective because it rapidly loses activity in culture media due to its hydrophobic nature,...

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Publicado en:Nat Commun
Main Authors: Tüysüz, Nesrin, van Bloois, Louis, van den Brink, Stieneke, Begthel, Harry, Verstegen, Monique M. A., Cruz, Luis J., Hui, Lijian, van der Laan, Luc J. W., de Jonge, Jeroen, Vries, Robert, Braakman, Eric, Mastrobattista, Enrico, Cornelissen, Jan J., Clevers, Hans, ten Berge, Derk
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group 2017
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5343445/
https://ncbi.nlm.nih.gov/pubmed/28262686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms14578
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