Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells

Wnt signalling proteins are essential for culture of human organ stem cells in organoids, but most Wnt protein formulations are poorly active in serum-free media. Here we show that purified Wnt3a protein is ineffective because it rapidly loses activity in culture media due to its hydrophobic nature,...

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Pubblicato in:Nat Commun
Autori principali: Tüysüz, Nesrin, van Bloois, Louis, van den Brink, Stieneke, Begthel, Harry, Verstegen, Monique M. A., Cruz, Luis J., Hui, Lijian, van der Laan, Luc J. W., de Jonge, Jeroen, Vries, Robert, Braakman, Eric, Mastrobattista, Enrico, Cornelissen, Jan J., Clevers, Hans, ten Berge, Derk
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2017
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5343445/
https://ncbi.nlm.nih.gov/pubmed/28262686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms14578
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