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A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins.

We have devised a strategy based on polymerase chain reaction (PCR) for the rapid cloning of functional antibody genes as single-chain immunotoxins. RNA from a hybridoma producing an antibody (OVB3) that reacts with ovarian cancer cells was used as a template to make the first strand of a cDNA. Then...

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Publicado no:Proc Natl Acad Sci U S A
Principais autores: Chaudhary, V K, Batra, J K, Gallo, M G, Willingham, M C, FitzGerald, D J, Pastan, I
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1990
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53411/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2105495/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.3.1066
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