A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins.
We have devised a strategy based on polymerase chain reaction (PCR) for the rapid cloning of functional antibody genes as single-chain immunotoxins. RNA from a hybridoma producing an antibody (OVB3) that reacts with ovarian cancer cells was used as a template to make the first strand of a cDNA. Then...
Gorde:
| Argitaratua izan da: | Proc Natl Acad Sci U S A |
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| Egile Nagusiak: | , , , , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
National Academy of Sciences
1990
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53411/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2105495/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.3.1066 |
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