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Determination of the pK(a) of the N-terminal amino group of ubiquitin by NMR

Ubiquitination regulates nearly every aspect of cellular life. It is catalysed by a cascade of three enzymes and results in the attachment of the C-terminal carboxylate of ubiquitin to a lysine side chain in the protein substrate. Chain extension occurs via addition of subsequent ubiquitin molecules...

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Publicat a:Sci Rep
Autors principals: Oregioni, Alain, Stieglitz, Benjamin, Kelly, Geoffrey, Rittinger, Katrin, Frenkiel, Tom
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2017
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5333110/
https://ncbi.nlm.nih.gov/pubmed/28252051
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep43748
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